from PyQt4 import QtCore, QtGui
from PyQt4.QtCore import SIGNAL, SLOT, QObject, QVariant

class App(QtGui.QWidget):
  def __init__(self, parent=None):
    # всякая инициализация
    # self.trayMenu = QtGui.QMenu(self)
    # заполнить меню
    ico=QtGui.QIcon(':/img/libru_gr.svg') # ":" - из ресурса, иначе указать отн. путь
    self.tray=QtGui.QSystemTrayIcon(self)
    self.tray.setIcon(ico)
    self.tray.setContextMenu(self.trayMenu)
    QObject.connect(self.tray, SIGNAL("activated(QSystemTrayIcon::ActivationReason)"), self.onTray)

    self.setWindowIcon(ico) # app window icon